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Bioassay

QuantiChrom Urea Assay Kit

Aplicativo

  • Para determinação quantitativa de ureia e avaliação dos efeitos de drogas no metabolismo da ureia.

Características principais

  • Sensível e preciso. Use amostras de 5 μL. Faixa de detecção linear de 0,08 mg / dL (13 μM) a 100 mg / dL (17 mM) de uréia em ensaio de placa de 96 poços.
  • Simples e de alto rendimento. O procedimento envolve a adição de um único reagente de trabalho e incubação por 20 min. Pode ser prontamente automatizado como um ensaio de alto rendimento para milhares de amostras por dia.
  • Maior estabilidade e versatilidade do reagente. A formulação otimizada melhorou bastante a estabilidade do reagente e do sinal. Ensaio em cuvete ou placa de 96 poços.
  • Baixa interferência em amostras biológicas. Nenhum pré-tratamento é necessário. Os ensaios podem ser realizados diretamente em amostras biológicas brutas, ou seja, na presença de lipídios e proteínas.
quantichromtm-urea-assay-kit-bioassay
quantichromtm-urea-assay-kit-bioassay

Método

  •  OD520nm (Químico)

Amostras

  •  Soro, plasma, urina, leite, cultura de células / tecidos, lavagem broncoalveolar (BAL), alimentos, bebidas e meio ambiente

Espécies

  •  Tudo

Tamanho

  •  100 testes

Limite de detecção

  •  0,08 mg / dL (13 μM)

Validade

  •  12 meses

Mais detalhes

  •  Uréia é produzido principalmente no fígado e segregado pelos rins. A uréia é o principal produto final do catabolismo protéico em animais. É o principal veículo para a remoção da amônia tóxica do corpo. A determinação da uréia é muito útil para o clínico avaliar a função renal dos pacientes. Em geral, os níveis aumentados de ureia estão associados a nefrite, isquemia renal, obstrução do trato urinário e certas doenças extra-renais, por exemplo, insuficiência cardíaca congestiva, doenças hepáticas e diabetes. Níveis diminuídos indicam insuficiência hepática aguda ou podem resultar de terapia com fluidos parenteral muito vigorosa. Procedimentos simples, diretos e prontos para automação para medir a concentração de ureia ou BUN de nitrogênio da ureia no sangue em amostras biológicas estão se tornando populares em Pesquisa e Descoberta de Medicamentos. O kit de ensaio de ureia da BioAssay Systems foi projetado para medir a ureia diretamente em amostras biológicas sem qualquer pré-tratamento. O método de Jung aprimorado utiliza um reagente cromogênico que forma um complexo colorido especificamente com a uréia. A intensidade da cor, medida em 520 nm, é diretamente proporcional à concentração de uréia na amostra. A formulação otimizada reduz substancialmente a interferência de substâncias nas amostras brutas.

Para determinação quantitativa de ureia e avaliação dos efeitos de drogas no metabolismo da ureia.

 

  • Sensível e preciso, use amostras de 5 μl
  • Faixa de detecção linear de 0,08 mg / dl (13 μM) a 100 mg / dl (17 mM) de uréia em ensaio de placa de 96 poços
  • Simples e de alto rendimento
  • O procedimento envolve a adição de um único reagente de trabalho e incubação por 20 min
  • Pode ser prontamente automatizado como um ensaio de alto rendimento para milhares de amostras por dia
  • Método: OD520 nm (Químico)
  • Amostras: soro, plasma, urina, leite, cultura de células / tecidos, lavagem broncoalveolar (BAL), alimentos, bebidas e meio ambiente

Maior estabilidade e versatilidade do reagente. A formulação otimizada melhorou bastante a estabilidade do reagente e do sinal. Baixa interferência em amostras biológicas. Nenhum pré-tratamento é necessário. Os ensaios podem ser realizados diretamente em amostras biológicas brutas, ou seja, na presença de lipídios e proteínas. Ensaio em cuvete ou placa de 96 poços. A uréia é produzida principalmente no fígado e secretada pelos rins. A uréia é o principal produto final do catabolismo protéico em animais.

É o principal veículo para a remoção da amônia tóxica do corpo. A determinação da uréia é muito útil para o clínico avaliar a função renal dos pacientes. Em geral, os níveis aumentados de ureia estão associados a nefrite, isquemia renal, obstrução do trato urinário e certas doenças extra-renais, por exemplo, insuficiência cardíaca congestiva, doenças hepáticas e diabetes.

Níveis diminuídos indicam insuficiência hepática aguda ou podem resultar de terapia com fluidos parenteral muito vigorosa. Procedimentos simples, diretos e prontos para automação para medir a concentração de ureia ou BUN de nitrogênio ureico no sangue em amostras biológicas estão se tornando populares em Pesquisa e Descoberta de Drogas. O kit de ensaio de ureia da BioAssay Systems foi projetado para medir a ureia diretamente em amostras biológicas, sem qualquer pré-tratamento.

O método de Jung aprimorado utiliza um reagente cromogênico que forma um complexo colorido especificamente com a uréia. A intensidade da cor, medida em 520 nm, é diretamente proporcional à concentração de uréia na amostra. A formulação otimizada reduz substancialmente a interferência de substâncias nas amostras brutas.

 

QuantiChrom™ Zinc Assay Kit

DIZN-250 250
EUR 489
Description: For quantitative determination of zinc ion Zn2+ and evaluation of drug effects on zinc metabolism. Key Features: Sensitive and accurate. Uses 50 µL samples. Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible. Low interference in biological samples. No pretreatments are needed. Method: OD425nm. Samples: Serum, plasma, urine, saliva, food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.78 µg/dL (0.12 µM).

QuantiChrom Hemoglobin Assay Kit

65-DIHB-250 250 tests
EUR 473

QuantiChrom™ Boron Assay Kit

DBOR-100 100
EUR 339
Description: For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.

QuantiChrom™ TBARS Assay Kit

DTBA-100 100
EUR 249
Description: For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation. Key Features: Sensitive and accurate. Linear detection range: colorimetric assay 1 - 30 µM, fluorometric assay 0.1 - 1.5 µM MDA. Method: OD535nm, or FL530/550nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 80 min. Kit size: 100 tests. Detection limit: 0.1 µM.

QuantiChrom™ Biotin Assay Kit

DBIO-100 100
EUR 229
Description: For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM.

QuantiChrom™ Copper Assay Kit

DICU-250 250
EUR 479
Description: For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism. Key Features: Sensitive and accurate. Linear detection range 7 µg/dL (1.0 µM) to 300 µg/dL (47 µM) copper in 96-well plate assay. Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Method: OD359nm. Samples: Biological, environment, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 7 µg/dL (1.0 µM).

QuantiChrom™ Indole Assay Kit

DIND-100 100
EUR 199
Description: For quantitative determination of indole and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565nm. Samples: Bacterial growth media and other biological samples. Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 3 µM.

QuantiChrom™ Lipase Assay Kit

DLPS-100 100
EUR 469
Description: For quantitative determination of lipase enzyme activity. Key Features: Sensitive and accurate. Linear detection range 40 to 1600 U/L lipase activity in 96-well plate assay. Convenient and high throughput. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day. Method: OD412nm. Samples: Serum, plasma, saliva, urine etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 40 U/L.

QuantiChrom™ Cobalt Assay Kit

DCBT-100 100
EUR 509

QuantiChrom α-Amylase Assay Kit

DAMY-100 100
EUR 550.8
Description: Quantitative determination of α-amylase activity by colorimetric (595nm) method. Procedure: 10 min. Kit size: 100 tests. Detection limit: 10 U/L. Shelf life: 6 months. Shipping: ambient temp; storage: 4°C.

QuantiChrom™ Calcium Assay Kit

DICA-500 500
EUR 389
Description: For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca2+ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).

QuantiChrom™ Indican Assay Kit

DIDC-01K 1000
EUR 419
Description: For quantitative determination of indican (indoxyl sulfate) in urine samples. Key Features: Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 µmoles/L). Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Urine. Species: All. Procedure: Assay takes 10 min. Kit size: 1000 tests. Detection limit: 0.2 mg/dL.

QuantiChrom™ Indican Assay Kit

DIDC-100 100
EUR 309
Description: For quantitative determination of indican (indoxyl sulfate) in urine samples. Key Features: Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 µmoles/L). Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Urine. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.2 mg/dL.

QuantiChrom™ Ethanol Assay Kit

DIET-500 500
EUR 489
Description: For quantitative determination of ethanol and alcohol metabolism. Key Features: Sensitive and accurate. Detection range 0.04 - 4% alcohol in 96-well plate assay. Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD580nm (Chemical). Samples: Alcoholic beverages etc. Species: All. Procedure: Assay takes 10 min. Kit size: 500 tests. Detection limit: 0.04%.

QuantiChrom™ Glucose Assay Kit

DIGL-100 100
EUR 319
Description: For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum and plasma samples. Method: OD630nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.7 mg/dL (39 µM).

QuantiChrom™ Phytase Assay Kit

DPHT-100 100
EUR 399
Description: A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples. Key Features: Simple and convenient: No complex detection reagents to mix. High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay. Fast and high-throughput: Homogeneous "mix-and-measure" assay allows for quantification of phytase activity within 60 minutes. Method: OD620nm. Samples: Enzyme extracts, agriculture and biological samples. Species: All (e.g. Wheat, Barley). Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

QuantiChrom™ Sulfate Assay Kit

DSFT-200 200
EUR 379
Description: For quantitative determination of sulfate ion and evaluation of drug effects on sulfate metabolism. Key Features: Sensitive and accurate. Detection range 0.02 mM (0.19 mg/dL) to 2 mM (19.2 mg/dL) sulfate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Method: OD600nm. Samples: Serum, urine, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 200 tests. Detection limit: 20 µM.

QuantiChrom™ Protein Assay Kit

QCPR-500 500
EUR 249
Description: For quantitative determination of total protein. Key Features: Sensitive and accurate. Use 10 mL samples. Detection range 0.06 - 1.0 mg /mL protein in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (<0.1%) and SDS (< 0.1% SDS) do not interfere in the assay. Versatility: assays can be executed in 96-well plate or cuvet. Method: OD595nm (Bradford). Samples: Biological, food, agriculture etc. Species: All. Procedure: Assay takes 2 min. Kit size: 500 tests. Detection limit: 60 µg/mL.

QuantiChrom™ Carbonyl Assay Kit

DCAR-100 100
EUR 349
Description: For quantitative determination of carbonyl groups or protein carbonyls and evaluation of drug modulators. Key Features: Fast and sensitive. Use of 100 µL sample. Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD375nm. Samples: Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 12 µM.

QuantiChrom™ Chromium Assay Kit

DCRM-250 250
EUR 459
Description: For quantitative determination of chromium Cr(VI). Key Features: Sensitive and accurate. Linear detection range of 20 - 2000 µg/L Chromium. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Biological (serum, plasma etc), environmental (water, soil etc), food and beverage samples. Species: All. Procedure: Assay takes 20 min. Kit size: 250 tests. Detection limit: 20 µg/L (0.38 µM, 0.02 ppm).

QuantiChrom™ Chloride Assay Kit

DICL-250 250
EUR 329
Description: For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl- in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD610nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.7 mg/dL (0.2 mM).

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